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poly a enrichment rna sequencing plasmidsaurus  (Plasmidsaurus)
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Plasmidsaurus poly a enrichment rna sequencing plasmidsaurus
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Poly A Enrichment Rna Sequencing Plasmidsaurus, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poly a enrichment rna sequencing plasmidsaurus - by Bioz Stars, 2026-10
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poly a selected rna sequencing  (Macrogen)
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Macrogen poly a selected rna sequencing
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Poly A Selected Rna Sequencing, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/kit+library+mrna+prep+stranded+truseq/pm42265539-69-33-29
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poly a selected rna sequencing - by Bioz Stars, 2026-10
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nebnext ultra iitm directional rna library prep with poly a mrna isolation kit  (New England Biolabs)
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New England Biolabs nebnext ultra iitm directional rna library prep with poly a mrna isolation kit
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Nebnext Ultra Iitm Directional Rna Library Prep With Poly A Mrna Isolation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/NEBNext+Poly(A)+mRNA+Mag+Isolation+Mod/pmc13158664-121-7-19
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nebnext ultra iitm directional rna library prep with poly a mrna isolation kit - by Bioz Stars, 2026-10
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poly a rna enrichment  (Novogene)
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Novogene poly a rna enrichment
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Poly A Rna Enrichment, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/sequencing/bio_rxiv__64898__2026__05__15__725290-74-0-11
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poly a rna enrichment - by Bioz Stars, 2026-10
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e7490 nebnext ultra ii directional rna library prep kit for illumina neb  (New England Biolabs)
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New England Biolabs e7490 nebnext ultra ii directional rna library prep kit for illumina neb
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
E7490 Nebnext Ultra Ii Directional Rna Library Prep Kit For Illumina Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e7490 nebnext ultra ii directional rna library prep kit for illumina neb - by Bioz Stars, 2026-10
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nebnext ultra ii directional rna library prep kit  (New England Biolabs)
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New England Biolabs nebnext ultra ii directional rna library prep kit
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Nebnext Ultra Ii Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/NEBNext+Poly(A)+mRNA+Mag+Isolation+Mod/10__1016_slash_j__cej__2026__177121-51-12-23
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nebnext ultra ii directional rna library prep kit - by Bioz Stars, 2026-10
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poly a rna seq libraries  (Novogene)
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Novogene poly a rna seq libraries
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Poly A Rna Seq Libraries, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poly a rna seq libraries - by Bioz Stars, 2026-10
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nonstranded poly a selected rna library preparations  (Novogene)
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Novogene nonstranded poly a selected rna library preparations
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Nonstranded Poly A Selected Rna Library Preparations, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/99+libraryies+polya+rna+selected+sequencing/pmc13143122-253-8-5
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nonstranded poly a selected rna library preparations - by Bioz Stars, 2026-10
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nebnext poly a rna magnetic isolation module 667  (New England Biolabs)
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New England Biolabs nebnext poly a rna magnetic isolation module 667
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Nebnext Poly A Rna Magnetic Isolation Module 667, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/NEBNext+Poly(A)+mRNA+Mag+Isolation+Mod/pm42045289-322-24-32
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nebnext poly a rna magnetic isolation module 667 - by Bioz Stars, 2026-10
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nebnext poly a rna magnetic isolation module  (New England Biolabs)
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New England Biolabs nebnext poly a rna magnetic isolation module
Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are <t>RNA</t> samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking <t>sequence,</t> identified in Supplemental Figure 2.
Nebnext Poly A Rna Magnetic Isolation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a+rna/NEBNext+Poly(A)+mRNA+Mag+Isolation+Mod/pm42011786-68-39-45
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Image Search Results


Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are RNA samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking sequence, identified in Supplemental Figure 2.

Journal: bioRxiv

Article Title: Human vault RNAs exhibit diverse expression patterns and inter-locus compensation

doi: 10.64898/2026.06.17.732918

Figure Lengend Snippet: Quantification of vtRNA1-1 (red), 1-2 (blue), 1-3 (green), and 2-1 (purple) relative expression across HEK293T cell lines via RT-qPCR. Values correspond to ΔΔC q , which is calculated as vtRNA C q normalized to GAPDH, then knockout ΔC q normalized to wild-type (“HEK293T”) for each paralog. Data points are RNA samples from independent biological replicates, and error bars indicate mean ± standard deviation. Dashed grey line is a visual aid for no expression change, and dotted grey lines are a composite statistical threshold (p<0.05 in a two-sample t-test, assuming n=3 per group and variances equivalent to the “HEK293T” samples). Corresponding Northern blots are in Supplemental Figure 4. * Gene has a small, off-target deletion in its flanking sequence, identified in Supplemental Figure 2.

Article Snippet: To probe for more subtle vtRNA-knockout effects, we isolated total RNA samples from our wild-type (HEK293T), CTRLdel, vt1-1 KO, vt1-2 KO, vt1-3 KO, and vt1-TKO cell lines and submitted them for (poly-A enrichment) RNA sequencing (Plasmidsaurus).

Techniques: Expressing, Quantitative RT-PCR, Knock-Out, Standard Deviation, Northern Blot, Sequencing

[a] Principal components analysis (PCA) of RNA-seq transcript counts per million transcripts (CPM) data for each HEK293T cell line. Data points correspond to RNA samples from independent biological replicates. [b] Volcano plots of transcripts differentially expressed between genetically edited cell lines and wild-type (WT) HEK293T. Upregulated (blue) and downregulated (red) transcripts have FDR < 0.05 (false discovery rate), and log 2 |FC| ≥ 1 (fold change), and their numbers are reported (“n”) for each differential expression analysis. The GAPDH and UBC transcripts (“Housekeeping”, purple) are shown as controls. Transcripts encoding proteins associated with vtRNA binding (“vtRNA Binders”, green) correspond to proteins previously identified by vtRNA pulldown .

Journal: bioRxiv

Article Title: Human vault RNAs exhibit diverse expression patterns and inter-locus compensation

doi: 10.64898/2026.06.17.732918

Figure Lengend Snippet: [a] Principal components analysis (PCA) of RNA-seq transcript counts per million transcripts (CPM) data for each HEK293T cell line. Data points correspond to RNA samples from independent biological replicates. [b] Volcano plots of transcripts differentially expressed between genetically edited cell lines and wild-type (WT) HEK293T. Upregulated (blue) and downregulated (red) transcripts have FDR < 0.05 (false discovery rate), and log 2 |FC| ≥ 1 (fold change), and their numbers are reported (“n”) for each differential expression analysis. The GAPDH and UBC transcripts (“Housekeeping”, purple) are shown as controls. Transcripts encoding proteins associated with vtRNA binding (“vtRNA Binders”, green) correspond to proteins previously identified by vtRNA pulldown .

Article Snippet: To probe for more subtle vtRNA-knockout effects, we isolated total RNA samples from our wild-type (HEK293T), CTRLdel, vt1-1 KO, vt1-2 KO, vt1-3 KO, and vt1-TKO cell lines and submitted them for (poly-A enrichment) RNA sequencing (Plasmidsaurus).

Techniques: RNA Sequencing, Quantitative Proteomics, Binding Assay